Designed for long-term live cell imaging and time-lapsed microscopy
Can be used with Petri dishes, chambered coverglasses, coverslip holders and glass bottom dishes, both 35 and 50 mm. Incorporates luer-lock ports for gas mixture (to control CO2 or hypoxia), inside reservoir for water to control humidity
Requires a heated cover (purchased separately), to prevent condensation
Built-in multiple ports for tubing and accessories (probes and sensors). Requires a temperature controller. Incorporates STAGE sensor for stable temperature control, and can be used with optional BATH probe to monitor inside temperature
Fits any microscope (specify microscope model when ordering, ships installed on the microscope adapter; some (Zeiss for example) microscopes need a recessed down insert - type K - and a different incubator with 160x110 mm foot print). Can be upgraded with a heated lid with openings to access cells with micro-injection tools to perform cell manipulation
Can be upgraded with an objective heater for immersion optics. Can be used with multi-channel solution switch and perfusion systems. An optional set of adjustable tubing holders MH-MIS allows you to position inflow and outflow tubing for continuous media exchange. All incubators require a lid, purchased separately
Low-Profile configurations (/L) are only 25 mm high and can be used with microscope condensers that require low vertical positioning to obtain bright-field images with Phase-contrast or DIC optics. Incorporates two ports for CO2 input and to replenish water to control humidity inside. Although this configuration provides two ports that can be used for tubing, it is not recommended for perfusion applications due to reduced vertical clearance inside
Incubator with 22x46 mm aperture, TC-MIS-20x46/L. Can be used with 50 mm dishes and chambers, slides / coverglasses, and, in combination with 50 mm reducing insert TC-PA50 (-C, -N, -F, -W), with 35 dishes and coverslip holders CSC / UTIC
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Specifications:
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Stability:
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0.1°C
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Outside dimensions:
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110 x 110 mm; 34 mm from sample plane to the top cover (25 mm clearance for low-profile configuration)
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Optical aperture (on the bottom):
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20x46 mm, 65x75 mm, 40, 35, 30, 15 and 11 mm diameter
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Use with:
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50 mm and 35 mm glass bottom dishes, 1x3 in. coverglasses / slides, coverslip holders CSC, custom devices, and UTIC chambers
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Condensation free lid:
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Uses a built-in temperature sensor to connect independently to the second channel of the temperature controllers; 70x70 mm optical window
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CO2 port:
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Luer-lock connector to a source of gas mixture.
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Water reservoir:
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Controls humidity. Can be replenished through outside ports.
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Media exchange and perfusion:
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Multiple sealed inflow / outflow ports, a set of optional adjustable tubing holders to position inflow and outflow tubing inside sample chambers.
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Perfusion cover low-profile, with perfusion ports and tubing, FLOW-LOW
For use inside TC-MIS incubators. Includes x2 sets of tubing to fit into reduced inflow and outflow ports, which extend to the bottom of a Petri dish. Tubing has a luer-lock fitting attached, and can be put through side ports of the incubator. This cover, if placed on a standard Petri dish, forms a flow cell. The high optical quality glass window on top is 12 mm diameter. Can be used in combination with PDI insert to provide laminar flow. Can be used to replenish media around your samples during time-lapse imaging. Can be sterilized with ethanol solution or autoclaved (100°C max).
Click on catalog numbers below to purchase online
Sample publications:
4 CbtA toxin of Escherichia coli inhibits cell division and cell elongation via direct and independent interactions with FtsZ and MreB; PLOS Genetics September 20, 2017
3 Bacillus subtilis chromosome organization oscillates between two distinct patterns. September 2, 2014 vol. 111 no. 35;
2 Molecular Architecture of Synaptic Actin Cytoskeleton in Hippocampal Neurons Reveals a Mechanism of Dendritic Spine Morphogenesis. Mol. Biol. Cell January 1, 2010 vol. 21 no. 1 165-176;
1 Evolution of Network Synchronization during Early Epileptogenesis Parallels Synaptic Circuit Alterations. The Journal of Neuroscience, 8 July 2015, 35(27): 9920-9934;
