Designed for live cell imaging and time-lapsed microscopy
The controller is used to deliver gas mixture inside any custom enclosure and miniature incubators. Connects to a cylinder with compressed pre-mixed (5% CO2 / 95% O2 or any other, including nitrogen and low O2) gas. The output connects directly to a gas port of the incubator or a humidifier. The controller regulates gas output to provide continuous slow stream of gas mixture, to replace residual gases inside the incubator, up to 750 SCCM maximum gas flow rate. Simple to use. Can be adjusted to fill incubators of different volumes. Balanced CO2 content inside the incubator controls pH of cell media
Note: if using a source of pure CO2, a different CO2-MI controller is required
Sample publications:
1. Micro Three-Dimensional Neuronal Cultures Generate Developing Cortex-Like Activity Patterns; Front. Neurosci., 02 October 2020
2. Variability of seizure-like activity in an in vitro model of epilepsy depends on the electrical recording method; Heliyon. 2020 Nov; 6(11)
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Specifications:
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Input:
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max 150 PSI
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Output:
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max 750 SCCM flow rate
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Connectors:
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Push-to-connect:
INPUT for 5/32 in. (4 mm) O.D. tubing (included);
OUTPUT for 1/8 in O.D. tubing (included);
can be replaced with any 10-32 threaded connectors;
includes luer fitting for different size tubing;
includes easy-disconnect coupling for 1/4 in. O.D. tubing
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Indicators:
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Digital display
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Controls:
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INPUT PRESSURE manual regulator;
Digital display
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Dimensions:
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8 x 4 x 9 in.
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Power:
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100-240VAC 35W
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Heated humidifier CO2-500 ml (Optional accessories)
A heated platform with 1L reservoir to provide gas saturation with water vapors by passing the gas mixture through heated water. When passed through heated water, the mixture becomes humid. Can be also used as an indicator of gas flow - bubbles will indicated gas flowing inside the incubator. Connects inline between the controller and the incubator
Required accessories: a source of CO2 gas, miniature incubator
Optional accessories: a humidifier CO2-500ML, small volume delivery system, gas mixture adapter, pressurized cylinders
