Miniature Incubator for standard microscopes to use with slides and Petri dishes, TC-MIS
Can be used with petri dishes, chambered coverglasses, coverslip holders and glass bottom dishes, both 35 and 50 mm. Incorporates luer-lock ports for gas mixture (to control CO2 or hypoxia), inside reservoir for water to control humidity, and heated cover, to prevent condensation. Built-in multiple ports for tubing and accessories (probes and sensors). Requires a temperature controller. Incorporates STAGE sensor for stable temperature control, and can be used with optional BATH probe to monitor inside temperature. Fits any microscope (specify microscope model when ordering, ships installed on the microscope adapter; some Zeiss microscopes need a recessed down insert - type K - and a different incubator with 160x110 mm foot print). Can be upgraded with a heated lid with openings to access cells with micro-injection tools to perform cell manipulation
Can be upgraded with an objective heater for immersion optics. For use with long-distance objectives, select TC-MIS-65x75-HB incubator with closed 1 mm heated glass bottom, which allows you to use both slides and Petri dishes. Can be used for long-term imaging. Can be used with multi-channel solution switch and perfusion systems. An optional set of adjustable tubing holders MH-MIS allows you to position inflow and outflow tubing for continuous media exchange. All incubators require the lid purchased separately
Low-Profile configurations ( /L) are only 25 mm high and can be used with microscope condensers that require low vertical positioning to obtain bright-field images with Phase-contrast and DIC optics. Incorporates two ports for CO2 input and to replenish water to control humidity inside. Although this configuration provides two ports that can be used for tubing, it is not recommended for perfusion applications due to reduced vertical clearance inside
Incubator with 22x46 mm bottom window, TC-MIS-20x46/L. Can be used with 50 mm dishes and chambers, slides / coverglasses, and, in combination with 50 mm reducing insert TC-PA50 (-C, -N, -F, -W), with 35 dishes and coverslip holders CSC/UTIC
Incubator with 35 mm bottom window for use with Petri dishes and coverslip holder CSC and UTIC, TC-MIS-35/L. Has the whole bottom open for easy access with immersion optics
Incubator with 15 mm bottom window for use with Petri dishes, TC-MIS-15/L. Provides heat from the bottom. Might require reducing insert TC-PA-C/F/N/W for different brands of petri dishes
Incubator with 11 mm bottom window for use with Petri dishes, TC-MIS-11/L. Provides heat from the bottom. Might require reducing insert TC-PA-C/F/N/W for different brands of Petri dishes
Incubator with 45 mm bottom window for use with 50 mm Petri dishes and chambers, TC-MIS-45/L. If used with 50 mm reducing insert TC-PA50, can be also used with 35 mm dishes and coverslip holders CSC and UTIC
Incubator with 30 mm bottom window for use with 50 mm Petri dishes and chambers, TC-MIS-30/L. Provides heat from the bottom. If used with 50 mm reducing insert TC-PA50, can be also used with 35 mm dishes and coverslip holders CSC and UTIC.
Incubator with 80x70 mm glass 1 mm thick heated closed bottom, TC-MIS-65x75-HB/L. For use with long-distance objectives only. Has the whole bottom closed with 1 mm glass to provide uniform heating. Can be used custom devices and UTIC chambers
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Specifications:
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Stability:
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0.1°C
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Outside dimensions:
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110 x 110 mm; 34 mm from sample plane to the top cover (25 mm clearance for low-profile configuration)
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Optical window (on the bottom):
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20 x 46 mm, 65 x 75 mm, 40, 35, 30, 15 and 11 mm diameter
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Use with:
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50 mm and 35 mm glass bottom dishes, and 1x3 in. coverglasses / slides, coverslip holders CSC, custom devices, and UTIC
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Condensation free lid:
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Uses a built-in temperature sensor to connect independently to the second channel of temperature controllers; 70 x 70 mm optical window
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CO2 port:
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Luer-lock connector to a source of gas mixture.
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Water reservoir:
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Controls humidity. Can be replenished through outside ports.
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Media exchange and perfusion:
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Multiple sealed inflow / outflow ports, a set of optional adjustable tubing holders to position inflow and outflow tubing inside sample chambers.
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Perfusion cover low-profile, with perfusion ports and tubing, FLOW-LOW
For use inside TC-MIS incubators. Includes x2 sets of tubing to fit into reduces inflow and outflow ports, which extend to the bottom of a petri dish. Tubing has a luer-lock fitting attached, and can be put through side ports of the incubator. This cover, if placed on a standard Petri dish, forms a flow cell. The high optical quality glass window on top is 12mm diameter. Can be used in combination with PDI insert to provide laminar flow. Can be used to replenish media around your samples during time-lapse imaging. Can be sterilized with ethanol solution or autoclaved (100°C max).
Click on catalog numbers below to purchase online.
Required accessories: temperature controller, microscope adapter, CO2 controller.
Optional accessories: objective heater, perfusion system and accessories, flow control.
Download PDF manual.
