Pressure Systems for Ejection of Picolitre Volumes in Cell Research

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The PicoSpritzer® III is a self-contained rack-mountable system which supplies repeatable pressure pulses. Intracellular applications range from femtolitre ejections of RNA into 50 μm diameter cells, up to nanolitre ejections into oocytes. Extracellular applications range from delivery of picolitre volumes of neuroactive substances to neurons during intracellular recording up to chemical stimulus delivery to complete organisms

The heart of the PicoSpritzer is its special high speed valve offering precise control of ejection volumes and millisecond range ejection times. Although volumes ejected are linear with both pressure and time the pulse duration has a greater dynamic range with more accurate and reproducible settings. This pulse duration is likely to be changed frequently during the course of an experiment while once established, pressure settings will be changed relatively little on a dailybasis

The pulse duration is controlled by a 3-digit thumbwheel switchable to read in milliseconds, seconds or minutes. A pulse may be initiated from a front panel button, a remote push-button, footswitch or external TTL pulse