Designed for long-term live cell imaging and time-lapsed microscopy
For use with any chamber or plate. Standard multi-well 86x128 mm plates will fit inside. Incorporates heated glass bottom
Requires a heated lid (purchased separately) to prevent condensation and to control CO2 or hypoxia
Built-in multiple ports for tubing and accessories, probes and sensors. One removable side provides multiple openings for micro-tubing. The chambers and devices can be formed directly on the glass bottom. Fits all brands of motorized stage (see table below). Can be used with a set of adjustable tubing holders to position perfusion tubing for continuous media exchange, provided optional inserts TC-I-100 or TC-I-4/3 are placed inside (see parts table at the bottom). The bottom is closed (for open bottom incubator, to access with immersion objective - consider another model)
Sample publications:
1 On-site genetic analysis for species identification using lab-on-a-chip. Ecol Evol. 2021 Feb; 11(4): 1535–1543
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Brand
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extension (if required)
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Ludl, Prior, ASI, Marhauser, Zeiss
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no extension required
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Nikon 236x155 mm:
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TC-MI-NIK
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Thorlabs 170x130 mm:
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TC-MI-THOR
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Ludl Bioprecision II, 172x116 mm:
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TC-MI-LUDL
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Specifications:
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Outside dimensions:
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fits 160x110 mm cutout of motorized stages; the bottom is recessed 11 mm below the top surface of motorized stages (8 mm below the mounting surface, comes with 3 mm spacers to reduce this recessed profile to 5 mm), and can be leveled by set screws positioned in the corners of the incubator base
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Inside dimensions:
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86x128 mm; 33 mm from bottom to top lid surface (23.5 mm for ultra-low lid option); 24 mm inside clearance (17.5 mm for ultra-low lid option); optional spacers allow to elevate the top surface 9.5 mm - to provide more space inside the incubator
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Heated bottom:
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1 mm glass
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Stability:
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0.01°C
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Use with:
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custom devices and chambers, standard multi-well plates, 35 mm dishes, and 1x3 in. glass coverglasses / slides
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Condensation free lid:
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Uses a built-in temperature sensor to connect independently to the second channel of temperature controllers, 81x121 mm optical window
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CO2 port:
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Luer-lock connector to a source of gas mixture.
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Media exchange and perfusion:
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Multiple sealed inflow ports, a set of adjustable tubing holders to position inflow and outflow tubing inside sample chambers.
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A set of tubing with luer-lock fitting and stainless steel needle attached x8, PS-KIT
Can be used with adjustable holders MH-MIS to provide inflow and outflow to chambers and dishes placed inside incubator. The holders are attached to optional inserts TC-I-4/3 or TC-I-100. Tubing fits through openings on sides of the incubator. The stainless needle attached to one end of the tubing can be bent to fit inside chambers and dishes. Includes multiple fitting (total more than 100 pieces), and threaded barbed luer locks for the incubator ports
For use with perfusion or continuous media exchange systems