Designed for long-term live cell imaging and time-lapsed microscopy
It can be used with standard multi-well plates and optional inserts: TC-I-20x30, TC-I-30x50, TC-I-SL for slides and micro-fluidics devices, TC-I-35 / TC-I-60 for standard 35 mm Petri dishes and for larger dishes (up to 60 mm diameter), TC-I-3 for slides and chambered coverglasses (x3), and TC-I-4 for CSC coverslip holders and Petri dishes (x4). The inserts can be used with a set of adjustable tubing holders MH-MIS to position sensors or perfusion tubing for continuous media exchange. Requires a heated cover (purchased separately) to prevent condensation and to control CO2 or hypoxia. Built-in multiple ports for tubing and accessories, probes and sensors. Fits all brands of motorized stages (Ludl, Prior, ASI, Marhauser) and type K Zeiss / Leica stages. Some models of motorized stages from Nikon, ThorLabs and Ludl might require optional adapter extensions to fit (see the table below). Requires a temperature controller. The whole bottom is open for access with a microscope objective (for closed heated bottom incubator consider TC-MWPHB model). Can be upgraded with an objective heater for immersion optics
Sample publications:
4 Integrated functional neuronal network analysis of 3D silk-collagen scaffold-based mouse cortical culture. STAR Protocols Volume 2, Issue 1, 19 March 2021, 100292;
3 In vivo dynamics of acidosis and oxidative stress in the acute phase of an ischemic stroke in a rodent model. j.redox.2021.102178;
2 A Human Organotypic Microfluidic Tumor Model Permits Investigation of the Interplay between Patient-Derived Fibroblasts and Breast Cancer Cells. Cancer Research April 16, 2019;
1 Breast Cancer Cell Invasion into a Three Dimensional Tumor-Stroma Microenvironment. Sci Rep. 2016; 6: 34094.;
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Brand
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extension (if required)
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Ludl, Prior, ASI, Marhauser, Zeiss
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no extension required
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Nikon 236x155 mm:
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TC-MI-NIK
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Thorlabs 170x130 mm:
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TC-MI-THOR
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Ludl Bioprecision II, 172x116 mm:
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TC-MI-LUDL
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Specifications:
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Outside dimensions:
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fits 160x110 mm cutout of motorized stages; the bottom is recessed 11 mm below the top surface of motorized stages (8 mm below the mounting surface, comes with 3 mm spacers to reduce this recessed profile to 5 mm), and can be leveled by set screws positioned in the corners of the incubator base
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Optical aperture and clearance:
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114x76 mm aperture; 33 mm from bottom to top lid surface (26.5 mm for ultra-low lid); 26 mm inside clearance (19.5 mm for ultra-low lid); optional spacers allow to elevate the top surface 9.5 mm - to provide more space inside the incubator
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Use with:
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Standard multi-well plates, 35-60 mm dishes, custom devices and 1x3 in. glass coverglasses / slides
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Stability:
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0.01°C
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Condensation free lid:
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Uses a built-in temperature sensor to connect independently to the second channel of temperature controllers, 81x121 mm optical window
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CO2 port:
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Luer-lock or barbed connector to a source of gas mixture.
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Media exchange and perfusion:
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Multiple sealed inflow/outflow ports, optional sets of adjustable tubing holders to position inflow and outflow tubing inside sample chambers.
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Magnetic insert, MA-128x86
This insert provides flexible working area for positioning accessories required for high resolution live sample imaging and recording: from media exchange and test solution delivery tubing, to sensors and electrodes. Specially treated stainless magnetic surface of the adapter provides ideal means to mount miniature adjustable magnetic holders. Shown on the picture are ZMM zero-dead volume manifold and MTH-S holder with stainless suction tubing. Incorporates adjustable clamps to fix all brands of 35 mm Petri dishes and CSC chambers, glass bottom dishes (both 35 and 50 mm), and heating elements
Click on image to enlarge
Insert for standard slides, TC-I-20x30
Designed to hold standard 1x3 in. slides. Provides wide access for fluidics tubing. Optical aperture is 20x30 mm, 1 mm thick lip to hold slides up to 76x26 mm. Includes adjustable clumps and thumb screws
Click on image to enlarge
Insert for slides and microfludics devices, TC-I-SL
Designed to position custom microfluidics devices and slides. Provides wide access for fluidics tubing - 80x70 mm recessed area. Optical aperture is 72x24 mm, 1 mm thick lip to hold slides up to 76x28 mm. Includes adjustable clumps and thumb screws
Click on image to enlarge
Insert for custom devices, TC-I-30x50
Designed to hold custom devices up to 101x51 mm. Provides wide access for custom accessories. Optical aperture is 50x30 mm. Includes adjustable clumps and thumb screws
Click on image to enlarge
Insert for 35 mm dishes, TC-I-35
Designed to position standard 35 mm Petri dishes. Provides wide access for custom accessories and fluidics tubing. Optical aperture is 25 mm. Includes adjustable clumps and thumb screws
Insert for 50-60 mm dishes, TC-I-60
Designed to position larger (up to 60 mm diameter) dishes. Provides wide access for custom accessories and fluidics tubing. Optical aperture is 30 mm. Includes adjustable clumps and thumb screws
Insert for x4 coverslip holders and Petri dishes, TC-I-4
Designed to hold x4 CSC coverslip holders and Petri dishes (up to 36.8 mm diameter) dishes. Some brands of Petri dishes require reducing rings (TC-PA-C, -F, -W, -N). Provides wide access for custom accessories. Optical aperture is 34 mm. Includes adjustable clumps and thumb screws
Insert for x3 slides / coverglasses, TC-I-3
Designed to hold x3 coverglasses and custom slides. Optical aperture is 70x20 mm
