Can be used for imaging and recording
Consists of a heated bottom base, and a silicone O-ring to seal the coverslip. The metal base facilitates heat transfer. There is no contact between solution and the chamber base to prevent ions leakage. The included top glass coverslip can be used to seal your sample from top as well - to from a micro-incubator. The top can be secured using the included metal ring, or using flat springs of microscope adapters. The bottom part has a recessed profile to fit round or square coverslips. The included O-rings allows you to use different thickness coverslips. Simply put the coverslip inside and seal it with silicone ring by a snap-in action, then secure with a top metal ring (the silicone ring can be also secured by flat springs of microscope adapters). For low-profile chambers, consider TC-CSC -L design, where no top clamps are required. Requires a microscope adapter and a temperature controller. 3-D Model
Sample publications.
Heated Chamber for replaceable round coverslips, low profile, TC-CSC -L
In contrast to regular CSC chambers, it does not need the top ring or top clamps to keep the assembly together. Instead, the silicone ring holds the coverslip leak-proof, which makes it low-profile
Consider adding a cover for CO2 / O2 control
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Specifications:
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Outside diameter:
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52 mm, fits 50 mm cutout of microscope adapters
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Height:
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8.5 mm (with top metal ring); 6mm for low profile
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Working volume:
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25 mm coverslip:
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21 mm, approx. 350 microl
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22x22 mm coverslip:
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19 mm, approx. 280 microl
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20 mm coverslip:
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16 mm, approx. 200 microl
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18 mm coverslip:
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14 mm, approx. 150 microl
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13 mm coverslip:
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9 mm, approx. 65 microl
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12 mm coverslip:
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8 mm, approx. 50 microl
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