Inside diameter is only 10 mm. The chamber has two separate openings for solution inflow and outflow to provide smooth perfusion and prevent bubbles from coming in. Low profile permits easy access with electrodes, pipettes and immersion optics. Combined with miniature holders, forms a perfusion system
Easy to use, simply release adhesive layer on the coverslip (even with media still present), and attach the chamber from the top. Coverslip is flashed on the bottom of the chamber
The chamber is made out of polycarbonate. It is only 5.5 mm thick (high). The bottom of the chamber is a replaceable 30 mm round coverslip. The coverslip is attached to the chamber by using adhesive layers (or any adhesive, even melted wax)
Easy to clean after use
Note: Can be used with any thickness 30 mm coverslips
The chamber fits inside microscope stage adapter, specify microscope model when ordering
The chamber can be rotated inside the stage to provide required orientation of the sample
Screw-type or magnetic tubing / electrode holders extend this flexibility even further. They can be positioned anywhere around the chamber, so that tubing is not in the way of objectives or recording electrodes. Furthermore, the holders can adjust the angle and, as a result, tubing can approach the sample from any direction. The holders can be used with perfusion manifolds. They also can be used to position reference electrodes or temperature probes, or even tubing to deliver gases, for example oxygen, over the sample. MTH-S stainless suction tubing provides smooth perfusion. If you are planning to use this system during perfusion experiments, all you need is a outflow unit for suction to remove solution during perfusion. Can be used for imaging and recording. Can be also used with 50 mm BD Falcon and Willco glass bottom dishes
Click on image to enlarge
Sample publication: 1
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Specifications:
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Outside diameter:
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36.7 mm
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Height:
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5.5 mm
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Working volume:
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10 mm diameter, approx. 100 microl
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