PolyAn has developed new standard reference materials to help correct and validate the performance of analytical instruments that detect, measure, and identify substances based on fluorescence:

• Fluorescence Calibration Slide
• Spectrum Calibration Microbeads and Spectrum Calibration Nanobeads
• Focus Beads
• Counting Beads

Fluorescence analytical procedures are increasingly being used in areas such as biotechnology, clinical diagnostics, drug development, and environmental monitoring, where standards for instrument qualification and method validation are important considerations

Spectrum Calibration Nanobeads – Fluorescent Nanoparticles for Calibration

Are highly photostable, multicolor fluorescent PMMA nanoparticles designed for calibration and performance validation of fluorescence detection systems, including flow cytometers, fluorescence microscopes, confocal microscopes, and super-resolution imaging platforms.

Ideal for **multicolor fluorescence applications and extracellular vesicle (EV) analysis**, these nanobeads support optical resolution assessment, fluorescence detection optimization, and colocalization studies.

Their refractive index is closer to that of extracellular vesicles compared to conventional polystyrene particles, making them particularly suitable for EV detection applications.

Features

• Particle sizes: 100–150 nm, 201–250 nm, and 475–525 nm.

• Excitation range: 365–650 nm.

• Emission range: 400–750 nm.

• Fluorescence: Multicolor, with 1, 2, or 4 populations.

• Surface functionalization: Carboxy, Aldehyde, Streptavidin, and Neutravidin.

• Material: Monodisperse PMMA with highly photostable fluorescent dyes.

• Applications: Flow cytometry, fluorescence microscopy, super-resolution imaging, and extracellular vesicle analysis.