Designed for live cell imaging and time-lapsed microscopy
For use with any custom enclosure and miniature incubators, to regulate CO2 level from 0 to 20%. Connects to a cylinder with compressed CO2 gas. The output connects directly to a gas port of the incubator or to a humidifier. Can provide up to 750 sccm gas flow. The controller continuously senses CO2 concentration inside the incubator through CO2-UP attachment, which is small enough (69x29x25 mm) to be placed inside custom enclosures. It can be also attached from outside. The controller also measures humidity inside the enclosure (0-100%). CO2 control at 5% level keeps pH of media constant
Simple to use: the controller ships calibrated for 5% level requirement. Can be adjusted to fill incubators and custom chambers of different volumes. The instrument not only controls gas content inside the incubator, but also brings the gas consumption to the minimum
Note: if using pre-mixed 5% CO2 / 95% O2 gas source - a different PC-MI controller is required
In order to control O2 concentration, hypoxia CO2-O2-MI or hyperoxia O2-CO2-N2-MI, another model is used
Sample publications.
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Specifications:
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Input:
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max 100 PSI
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Output:
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max 750 sccm gas flow
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Connectors:
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Push-to-connect: INPUT for 5/32 in. (4 mm) O.D. tubing (included); OUTPUT for 1/8 in O.D. tubing (included); can be replaced with any 10-32 threaded connectors; includes luer fitting for different size tubing; includes easy-disconnect coupling for 1/4 in. O.D. tubing
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Indicators:
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Digital display
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Controls:
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CO2 level 1-20%
INPUT PRESSURE manual regulator 0-25 PSI,
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Monitor:
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Digital display
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Dimensions:
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8 x 4 x 9 in. controller
69x29x25 mm - CO2 attachment
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Power:
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100-240VAC 75W
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Mixing Chamber, CO2-MIX
Designed to provide CO2 control inside small custom chambers and micro-incubators, where the small size does not allow mounting CO2-UP sensor attachment. The mixing chamber dimensions are 100x100x32 mm, which are small enough to fit on a microscope stage. The chamber has x4 ports to connect multiple micro-incubators, and one input from CO2 controller. CO2-UP can be attached to the side of the mixing chamber. After mixing to the required concentration (5% CO2 for example), the gas mixture can be pumped into the micro-incubator using an optional pump or the miniature flow controller, that can deliver from micro-liters to 22 ml / min gas flow.
Required accessories: a source of CO2 gas, CO2-UP upgrade, miniature incubator