Functionalized Coverslips

 

For high-resolution microscopic imaging or scanning applications PolyAn offers functionalized coverslips. All of PolyAn's standard functional coatings can also be applied to coverslip glass

All coverslips have the standard dimension of 25 x 60mm. Type #1.5 is used as a standard thickness. All coverslips are packed in boxes of 5. Other functionalities, thicknesses and sizes are available upon request. PolyAn also offers functionalised substrates that are coated with gold or other noble metals as well as waveguide materials

2D-functionalized Coverslips

Id Surface Modifications Product Dimensions Packaging Volume
104 00 026 2D-Amino1 25 x 60 mm 5 Coverslips/Box
104 00 126 2D-Carboxy2 25 x 60 mm 5 Coverslips/Box
104 00 226 2D-Epoxy3 25 x 60 mm 5 Coverslips/Box
104 00 326 2D-Aldehyde4 25 x 60 mm 5 Coverslips/Box
104 00 526 2D-Thiol5 25 x 60 mm 5 Coverslips/Box
104 00 626 2D-Azide6 25 x 60 mm 5 Coverslips/Box

1) 2D-Amino functionalized Type #1.5 coverslips

2) 2D-Carboxy functionalized Type #1.5 coverslips

3) 2D-Epoxy functionalized Type #1.5 coverslips

4) 2D-Aldehyde functionalized Type #1.5 coverslips

5) 2D-Thiol functionalized Type #1.5 coverslips

6) 2D-Azide functionalized Type #1.5 coverslips. For covalent coupling of Alkyne-or DBCO modified biochemical species via click-chemistry

For cost-sensitive applications PolyAn has developed a range of 2D (2-dimensional)-reactive coverslips that are manufactured from high quality glass with an ultra-flat surface and low inherent fluorescence. The glass is coated with a thin silane layer that will covalently bind most types of bio-molecules. The defect-free surface features uniform silane layers that provide a high covalent coupling efficiency together with a very low background

For DNA combing applications PolyAn also offers functionalized coverslips

3D-functionalized Coverslips

Id Surface Modifications Product Dimensions Packaging Volume
104 00 006 3D-Amino1 25 x 60 mm 5 Coverslips/Box
104 00 106 3D-Carboxy2 25 x 60 mm 5 Coverslips/Box
104 00 206 3D-Epoxy3 25 x 60 mm 5 Coverslips/Box
104 00 306 3D-Aldehyde4 25 x 60 mm 5 Coverslips/Box
104 00 407 3D-NHS5 25 x 60 mm 5 Coverslips/Box
104 00 506 3D-Thiol6 25 x 60 mm 5 Coverslips/Box
104 01 206 Poly-L-Lysin (covalently coated)7 25 x 60 mm 5 Coverslips/Box
104 01 306 3D-PDITC (Activated Amino)8 25 x 60 mm 5 Coverslips/Box
104 01 406 3D-Maleimide9 25 x 60 mm 5 Coverslips/Box
104 02 206 Streptavidin (covalently coated)10 25 x 60 mm 5 Coverslips/Box
104 03 206 Neutravidin (covalently coated)11 25 x 60 mm 5 Coverslips/Box

1) 3D-Amino functionalized Type #1.5 coverslips

2) 3D-Carboxy functionalized Type #1.5 coverslips

3) 3D-Epoxy functionalized Type #1.5 coverslips

4) 3D-Aldehyde functionalized Type #1.5 coverslips

5) 3D-NHS functionalized Type #1.5 coverslips

6) 3D-Thiol functionalized Type #1.5 coverslips

7) Poly-L-Lysin functionalized Type #1.5 coverslips. Poly-L-Lysin surfaces are well suited for coupling of biochemical species. Especially, when the storage buffer contains glycerol Poly-L-Lysin surfaces are an interesting alternative to reactive matrices

8) 3D-PDITC (Activated Amino) functionalized Type #1.5 coverslips

9) 3D-Maleimid functionalized Type #1.5 coverslips. Maleimide surfaces are used in „click chemistry“ for oriented covalent coupling (immobilization) of thiolated biochemical species

10) Covalently coated Streptavidin, Type #1.5 coverslips

11) Covalently coated Neutravidin, Type #1.5 coverslips

3D-Surfaces made by Molecular Surface Engineering

PolyAn’s 3D-functionalised microarray Slides are functionalized with a three-dimensional (3D)-surface chemistry comprised of a long-chain polymer containing a defined number of reactive groups. This polymer is covalently linked to the surface of the coverslip.

Our MSE-technology gently binds the functional layer onto the surface without damaging the base substrate. The morphology of the functional surface and thus the number of the reactive groups can be fine-tuned within a narrow range. This yields a number of advantages:

Low fluorescence background Covalent binding of functional layer on the substrate without changing the initial autofluorescence.
Low unspecific binding Combination of reactive functional groups with PolyAn antifouling matrix
Topography Tuneable surface
hydrophilicity / hydrophobicity (contact angle)
Uniform spot morphology Narrow variation of surface properties and homogeneous distribution of reactive groups
Optimal density and high accessibility of reactive groups Morphology and thickness of functional layer tailored to the desired application

 

Technical specifications

Packaging:

The coverslips are packaged in inert atmospheric boxes, with a capacity of 5 coverslips per box.

Storage

PolyAn’s coverslips are characterised by a long shelf-life when stored dry, at 4-8°C and protected from sunlight. All coverslips are packaged in boxes under Argon atmosphere to avoid contamination with particles. The Argon atmosphere also minimises degradation of the reactive surface through contact with air or humidity

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