category-fastwells-reagent-barriers

Fastwells and Silicone Isolators

FastWells™ Reagent Barriers

FastWells™ are sticky, flexible silicone gaskets that form hydrophobic reagent barriers around specimens without messy adhesives or special slides. Gaskets may be stacked to increase depth and volume

FastWell™ Reagent Barriers provide rapid isolation of cells and tissues on slides or coverslips for antibody incubations. The barriers remain sealed during agitated washing steps, preventing reagents from spilling when shaken or rocked. FastWells™ peel off quickly and cleanly, leaving no residue to interfere with specimen cover-slipping. They can be washed, autoclaved and reused. The barriers can also be sealed to form incubation chambers using flexible HybriSlip™ covers.

Applications

  • Microscopy
  • Fluorescence In situ Hybridization (FISH)
  • Single-molecule fluorescence analysis
  • Immunohistochemistry

Products

Id Title  
664112 FW9-FastWells 9mm Dia. X 1.0mm Depth - 18 X 18mm OD - 50 PACK1  
664113 FW20-FastWells 20mm Dia. X 1.0mm Depth / 25 X 25mm OD - 50 PACK2  
664114 FW1932-FastWells 32mm X 19mm ID X 1.0mm Depth, 25.5 X 44mm OD - 50 PACK3

1) FastWells Reagent Barriers are sticky, flexible silicone gaskets form hydrophobic reagent barriers around specimens without messy adhesives or special slides. Stackable and reusable

2) FastWells Reagent Barriers are sticky, flexible silicone gaskets form hydrophobic reagent barriers around specimens without messy adhesives or special slides. Stackable and reusable

3) FastWells Reagent Barriers are sticky, flexible silicone gaskets form hydrophobic reagent barriers around specimens without messy adhesives or special slides. Stackable and reusable

References

  • Yokomizo, T., & Dzierzak, E. (2010). Three-dimensional cartography of hematopoietic clusters in the vasculature of whole mouse embryos. Development, 137(21), 3651–3661
  • Yokomizo, T., Yamada-Inagawa, T., Yzaguirre, A. D., Chen, M. J., Speck, N. A., & Dzierzak, E. (2012). Whole-mount three-dimensional imaging of internally localized immunostained cells within mouse embryos.Nature Protocols, 7(3), 421–431
  • Wang, Y.-H., Collins, A., Guo, L., Smith-Dupont, K. B., Gai, F., Svitkina, T., & Janmey, P. A. (2012). Divalent Cation-Induced Cluster Formation by Polyphosphoinositides in Model Membranes. Journal of the American Chemical Society, 134(7), 3387–3395